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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 20: Poster Session I

CPP 20.57: Poster

Montag, 12. März 2018, 17:30–19:30, Poster A

Real-time XRR study of protein adsorption at the solid-liquid interface guided by multivalent ions — •Simon Schönberg¹, Madeleine Fries¹, Alexander Hinderhofer¹, Fajun Zhang¹, Robert M. J. Jacobs², Maximilian W. A. Skoda³, and Frank Schreiber¹ — ¹Institute for Applied Physics, University of Tübingen, 72076 Tübingen, Germany — ²Department for Chemistry, Chemistry Research Laboratory, University of Oxford, OX1 3TA, United Kingdom — ³Rutherford-Appleton Laboratory, ISIS Facility, Didcot

The unique influence of multivalent ions on the phase behaviour of protein solutions has been subject of past and current research [1]. Moreover, the influence of multivalent ions (Y³⁺) on the adsorption behaviour of protein (BSA) at the solid-liquid interface and thus the connection of bulk and interface behaviour was recently established [2]. In this context, we designed a new liquid cell for measurements at the solid-liquid interface via X-ray reflectivity (XRR). This setup allows real-time measurements of protein adsorption through the use of a molybdenum source. We successfully established this new XRR setup, which enables us to reproduce previous outcomes of ellipsometry measurements on multivalent ion-tuned protein adsorption. Further it allows to study more parameters such as temperature dependency of our system, surface roughness and surface coverage/density and thus promotes a more detailed understanding of protein adsorption tuned by multivalent ions.
Zhang, F. et al., Pure Appl. Chem (2014), 86, 191-202.
Fries, M. R. et al., Phys. Rev. Lett. (2017), in print.