Berlin 2018 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe
CPP: Fachverband Chemische Physik und Polymerphysik
CPP 33: Poster Session III
CPP 33.34: Poster
Dienstag, 13. März 2018, 14:00–16:00, Poster C
AC field assisted deposition of antibodies for virus detection — •Sandra Stanke1,2, Christian Wenger3, Frank Bier1,2, and Ralph Hoelzel1 — 1Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalysis and Bioprocesses IZI-BB, Potsdam, Germany — 2University of Potsdam, Germany — 3IHP GmbH, Leibniz Institute for Innovative Microelectronics, Frankfurt/Oder, Germany
Alternating current (AC) electrokinetics is a set of different effects that can be used for the manipulation of nano-particles in a non-uniform electric field. Here, we present an AC-electrokinetic driven sensor for the manipulation and movement of biological nanoparticles. This sensor consists of four individual arrays, each built up of more than 6000 nano-electrodes with a diameter of 500 nm each. Individually controlled functionalization of the sub-arrays is performed by AC electrokinetic forces: dielectrophoresis and AC electroosmosis. Consequently, molecules like antibodies can be attracted to the electrode surface. This allows the local concentration of the analyte at the sensor surface. Since these arrays can be addressed individually, each array can be functionalized with a different antibody. Thus, it is possible to detect, characterize and subtype different strains of the influenza virus. Additionally, each electrode is part of an on-chip resonant circuit, whose frequency changes with surface coverage of the electrode and, hence, serves as a measure of the amount of viruses bound to the antibody. The universal chip design does not limit the application to influenza viruses but also to different viruses, bacteria and parasites.