Berlin 2018 – wissenschaftliches Programm

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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 37: Focus: Smart Hydrogels and Hydrogel Based Devices II - organized by Gerald Gerlach, Walter Richtering and Thomas Hellweg

CPP 37.1: Topical Talk

Mittwoch, 14. März 2018, 09:30–10:00, C 130

Enzyme-functionalized polymer microgels for drug building block synthesis — •Julian Thiele — Leibniz-Institut für Polymerforschung Dresden e.V. / Leibniz Research Cluster, Dresden, Germany

In an attempt to close the current innovation gap in antibiotics research, we utilize highly specific, genetically engineered enzymes for drug synthesis in cell-free biotechnology. These enzymes require tailored experimental platforms that maintain enzyme activity, enhance the enzyme*s shelf life, and allow for extraction of valuable enzymes from reaction mixtures for recycling purposes. Being part of the Leibniz Research Cluster - an interdisciplinary network of five young investigator groups - we perform enzymatic reactions in polymer microgels with tailored size, porosity and functionalization. As material basis, we employ hyaluronic acid, which is cross-linked by mild [4+2]cycloaddition or thiol-Michael addition, and functionalized with Ni-NTA linker molecules to capture His-tagged enzymes. By loading these microgels into microreactors with active components (e.g. heating pads and valves), we tailor reaction conditions down to the micrometer scale and extract samples for in situ analytics by HPLC-MS and CARS as well as colorimetric assays, which are designed towards individual reaction intermediates and products. We show the capability of this approach using the example of malonate-to-Malonyl CoA conversion, which is a key intermediate in the synthesis of polyketide-based drugs.