Berlin 2018 – scientific programme
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CPP: Fachverband Chemische Physik und Polymerphysik
CPP 45: Charged Soft Matter, Polyelectrolytes and Ionic Liquids I
CPP 45.7: Talk
Wednesday, March 14, 2018, 12:45–13:00, C 243
Competing interactions and the formation of dynamic protein clusters — •Anita Girelli1, Michal K. Braun1, Felix Roosen-Runge2, Marco Grimaldo3, Tilo Seydel3, Fajun Zhang1, and Frank Schreiber1 — 1Institut für Angewandte Physik, Universität Tübingen, 72076 Tübingen — 2Physical Chemistry, Lund University, Sweden — 3ILL Grenoble, France
Protein clusters are potential precursors for protein crystallization.
The question of their formation and transient, dynamic or permanent nature is of
high relevance to structural biology and biomedicine. Here we report
on protein clusters in bovine serum albumin (BSA) - trivalent salt
(YCl3, LaCl3) systems. The protein clusters are most probably held
together by ion bridges and precede a condensation regime [1, 2]. The
clusters are characterized by dynamic light scattering (DLS). For a
molar ratio of salt concentration and protein concentration, cs /cp, equal to 4, we can observe the formation of clusters with a lifetime longer than 1
ms. Based on a comparison with self-diffusion data from neutron back scattering experiments [3], we assume that dynamic clusters form for cs /cp between 2 and
4. Furthermore, SAXS measurements for samples under these conditions
demonstrate the competing interactions, which support the formation
of dynamic clusters.
Soraruf et al., Soft Matter, 10, 894, 2014
Zhang et al., J. Appl. Cryst., 44, 755, 2011
Grimaldo et al., J. Phys. Chem. Lett., 6, 2577, 2015