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DS: Fachverband Dünne Schichten

DS 17: Poster Session I

DS 17.43: Poster

Dienstag, 13. März 2018, 18:15–20:15, Poster B

A biosynthetic pathway reconstituted within compartmentalized vesicles — •Akanksha Moga — Max Planck Institute of colloids and Interfaces, Potsdam, Germany

Cells are complex chemical systems composed of distinct compartments that function in a systematic manner. Compartmentalization of the cell is considered as a key step in the direction of eukaryotic evolution that accommodates multiple bio-chemical reactions. These compartments establish physical boundaries for biological processes that enable the cell to carry out different metabolic activities at the same time. In the discipline of bottom-up synthetic biology, lipid vesicles serve as an artificial cell but there is need to compartmentalize the processes within these vesicles. Here, we aim to mimic a cell by developing compartmentalized Giant Unilamellar Vesicles (cGUVs), as an artificial cell model.To illustrate the role of compartments, we study a multi-compartment enzymatic pathway, biosynthesis of molybdenum co-factor (Moco). Moco biosynthesis is highly conserved and ubiquitous pathway that takes place in a typical eukaryotic mitochondrion. The reactions involves cascade of protein/enzymatic reactions but protein encapsulation is not always supported by conventional lipid vesicles generation techniques. Therefore, we have employed an emulsion based phase transfer method to not only produce cGUVs but also to encapsulate specific substrates. Our preliminary results have shown promise with reproducibility of stable GUVs encapsulating various compounds and enzyme from Moco biosynthesis.