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KFM: Fachverband Kristalline Festkörper und deren Mikrostruktur

KFM 13: Diamond I

KFM 13.1: Hauptvortrag

Mittwoch, 14. März 2018, 09:30–10:00, E 020

Advanced Cell Adhesion of Modified Ultrananocrystalline Diamond Surfaces — •Cyril Popov — Institute of Nanostructure Technologies and Analytics, Center for Interdisciplinary Nanostructure Science and Technology (CINSaT), University of Kassel, Germany

The interactions of different types of cells with ultrananocrystalline diamond (UNCD) surfaces with H-, O- and NH2-terminations were investigated with respect to cell density and viability. No cyto- or genotoxicity, no adverse effects on the cell viability and physiological responses were detected. The observed fast and strong attachment of the cells was attributed to a favorable combination of topography, surface chemistry and wettability. The enhanced attachment of the neurons was especially advantageous for the immunocytochemical procedures where the cell losses during washing steps were significantly reduced by one order of magnitude. In addition, applying photolithography the integration of a titanium grid structure under the UNCD films allowed for individual assignment of physiologically characterized neurons. The patterning of the UNCD surface termination by a hard mask and two modification steps resulted in guided cell attachment and growth.