Berlin 2018 – scientific programme

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MM: Fachverband Metall- und Materialphysik

MM 27: Poster Session I

MM 27.30: Poster

Tuesday, March 13, 2018, 18:30–19:45, Poster E

Impact of UV light on the mechanical properties of collagen fibrils probed by Raman spectroscopy and Atomic Force Microscopy — •Marcus Schulze, Annika Stocker, Melanie Rogge, and Robert Stark — Physics of Surfaces, Materialwissenschaften, TU Darmstadt, Alarich-Weiss-Str. 16, 64287 Darmstadt

Collagen plays a decisive role in the development of substrates for tissue engineering. The controlled adjustment of its mechanical properties is essential and a potential strategy to achieve this is through physical treatment, such as UV light irradiation.

The focus of this study is to record the influence of heat and UV light on single collagen fibrils in situ. To that end, atomic force microscopy (AFM) was used for the simultaneous mapping of topography and modulus of type I collagen fibrils from rat tail tendon. The fibrils were probed in varied liquid environments (deionized water and phosphate buffered saline (PBS)) under UV light of varied wavelengths and temperatures of up to 45°C. Furthermore, structural changes in hydrated collagen films due to UV irradiation were investigated by means of Raman spectroscopy.

Our results showed that (UV/thermal) treatment performed in deionized water lowered the modulus of the collagen fibril. However, experiments performed in PBS-based solutions in combination with UV-B and UV-C light or thermal treatment of up to 45 °C showed an increase of the modulus within the first 30 to 40 minutes.