Erlangen 2018 – scientific programme

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P: Fachverband Plasmaphysik

P 17: Atmospheric Pressure Plasmas - Poster

P 17.8: Poster

Wednesday, March 7, 2018, 16:15–18:15, Zelt Ost

Biofilm Sterilization on Stainless steel by Synergistic Treatment of Atmospheric Pressure Plasma and Chelators — •Chen-Yon Tobias Tschang and Markus Thoma — I. Physikalisches Institut, Justus-Liebig-University Giessen, Germany

Atmospheric pressure plasma (APP) had been show to be strongly effective against bacteria. However, The efficiency drops dramatically when treating bacteria biofilm. Chelators, such as citric acid or ethylenediaminetetraacetic acid (EDTA), are organic compounds which bonds metal ions. Chelators are assumed to prevent or interrupt biofilm forming by bonding of calcium and magnesium ions. In this study, we investigate the synergistic effect of chelators and APP on E. coli biofilms on stainless steel plates. Chelators of EDTA and citric acid with different concentration were applied. The plasma source was a surface micro-discharge (SMD). Sterilization rates of different treatment time and concentration were investigated. In addition, a system of mist plasma were also utilized in order to examine the synergistic effects of chelator aerosols and APP. Live/Dead stain were applied for preventing viable but nonculturable (VBNC) state of bacteria. Results indicate that combination treatment of 7.5% of citric acid with plasma treatment increase the sterilization efficiency at least 10 times higher, while single treatment of citric acid had almost no effects. Mist plasma treatment did not result in significant change of sterilization efficacy.