München 2019 – wissenschaftliches Programm

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SYPP: Symposium Pulsed Power für Medizin und Biotechnologie

SYPP 2: Pulsed Power für Medizin und Biotechnologie II

SYPP 2.4: Hauptvortrag

Donnerstag, 21. März 2019, 15:30–16:00, HS 3

Spark discharges as tool for the extraction of microalgal compounds — •Katja Zocher, Raphael Rataj, Anna Steuer, and Juergen F Kolb — Leibniz Institute for Plasma Science and Technology, Felix-Hausdorff-Straße 2, 17489 Greifswald, Germany

Microalgae have gained in importance for green biotechnology during the last years. Besides their potential as feedstock for alternative renewable energy resources, they also contain valuable metabolites for pharmaceutical and nutritional applications, such as lipids, proteins, polysaccharides, and pigments.Although only frugal cultivation conditions are necessary, microalgae are distinguished by a robust cell wall, which allows a remarkable mechanical and chemical robustness. This characteristic often results in poor extraction yields. Conventional extraction techniques have shown to be therefore frequently energy and time consuming, which causes disproportional economic costs. Accordingly, major improvements of extraction technologies are necessary for successful commercialisation. In previous works [1][2], we could show that spark discharges, instigated directly in the microalgae suspension by 100-ns high voltage pulses, offer a gentle and yet effective approach. In comparison to a selected reference method, proteomic analysis revealed commonalities and differences in the protein distribution pattern, although the number of extracted proteins was the same and, in particular, valuable heat sensitive compounds could be extracted. Schlieren diagnostics and atomic forced microscopy was applied to elucidate the responsible spark characteristic for successful cell wall rupture. [1-2] Zocher, K. et al., 2016 and 2018 (under review)