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BP: Fachverband Biologische Physik
BP 1: Protein structure and dynamics
BP 1.3: Vortrag
Montag, 1. April 2019, 10:15–10:30, H4
Imaging single proteins with Low Energy Electron Holography — •Hannah Ochner1, Sven Szilagyi1, Sabine Abb1, Stephan Rauschenbach1,2, and Klaus Kern1,3 — 1Max-Planck-Institut für Festkörperforschung, Stuttgart — 2Chemistry Research Laboratory, Department of Chemistry, University of Oxford — 3École Polytechnique Fédérale de Lausanne
Recently, Low Energy Electron Holography (LEEH) has been shown to be able to image proteins at the single molecule level (without averaging), while avoiding radiation damage [1]. LEEH [2] is a lens-free imaging method in which the sample is radiated by coherent low energy electrons (50-200eV) [3] to form holograms that in principle contain full 3D information of the object. Basic Kirchhoff-Fresnel propagation-based numerical reconstruction of the holograms yields the shapes of the investigated proteins, as well as some internal contrast that already hints at 3D information. Thus, this technique can serve as a complementary method for protein structure determination, especially for types of proteins that are hard to access using other methods such as Cryo-EM or X-ray crystallography. We present the current state of the experiment and the reconstruction process along with future plans to enhance resolution and to improve the reconstruction towards including 3D information.
[1] PNAS 114, 1474-1479 (2017)
[2] Phy. Rev. Lett, 1990, 65(10), 1204-1206.
[3] Phys. Scr., 1988, 38, 260