Regensburg 2019 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 10: Crystallization, nucleation and self-assembly (joint session CPP/BP)

BP 10.2: Vortrag

Dienstag, 2. April 2019, 09:45–10:00, H14

Protein crystallization near liquid-liquid phase separation — Klim Petrov, Jan Hansen, •Florian Platten, and Stefan U. Egelhaaf — Heinrich Heine University Duesseldorf

The crystallization of protein (lysozyme) solutions is studied as a function of protein and salt concentration at ambient conditions. In addition to tetragonal crystals at low salt concentrations (far away from phase separation), needle-like and kinetically roughened crystals occur in the vicinity of the binodal. The crystallization induction time and the growth rate are inferred from optical microscopy and linked to the solubility and protein-protein interactions. Based on these data, the different states of the protein solution are linked to different driving forces for crystallization.