Regensburg 2019 – scientific programme

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BP: Fachverband Biologische Physik

BP 12: Poster II

BP 12.13: Poster

Tuesday, April 2, 2019, 14:00–16:00, Poster B2

Towards Label Free Imaging of Action Potentials by Deep Learning — •Stephan Rinner¹, Alberto Trentino¹, Heike Url², Bernhard Wolfrum², and Friedemann Reinhard¹ — ¹Walter Schottky Institut and Physik-Department, Technische Universität München, Am Coulombwall 4, 85748 Garching, Germany — ²Munich School of Bioengineering, Technische Universität München, Boltzmannstrasse 11, 85748 Garching, Germany

Imaging of action potential signals in cells thus far requires fluorescence labels. Interestingly, it is plausible that action potentials could also induce small intrinsic changes in the optical properties of a cell. We aim to develop a label-free imaging method by searching for such signatures, capitalizing on the emergence of powerful cameras and image recognition schemes over the past years. On this poster, I will present our efforts to resolve such signals using high resolution polarized light microscopy of heart cells. I will introduce the key features of our setup, namely high-powered LED illumination, a high framerate slow-motion camera and an incubation system for keeping cells alive. Furthermore, the poster will summarize data processing methods to identify patterns in small fluctuations, such as different filtering methods. If successful, this experiment will provide a new method to observe action potentials in a label-free and non-invasive manner.