Regensburg 2019 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 12: Poster II
BP 12.20: Poster
Dienstag, 2. April 2019, 14:00–16:00, Poster B2
Open-source 3D-printed Digital Inline Holographic Microscope for microscopic cell analysis — Stephan Amann1,2, Max von Witzleben1, and •Stefan Breuer1 — 1Institute of Applied Physics, Technische Universität Darmstadt, Schlossgartenstraße 7, 64289 Darmstadt, Germany — 2Department of Physics, Norwegian University of Science and Technology, Høgskoleringen 5, NO-7491 Trondheim, Norway
Digital inline holographic microscopy (DIHM) is a promising cellular object imaging modality. We demonstrate two cost-efficient DIHM experiments set-ups comprising of standard LED and semiconductor laser light sources and a Raspberry Pi Camera for image acquisition. The DIHM is 3D-printed yielding a highly compact and portable microscope. Imaging of cellular micro objects including tobacco cells and human red blood cells is performed by the use of open-source reconstruction software. Spatial resolutions of 3.96 micrometer and 1.98 micrometer are achieved. The developed DIHM is cost-efficient (<$200), compact and portable and constitutes a highly flexible tool to be used in science and education that can be tailored flexibly to the researchers demand.