Regensburg 2019 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 12: Poster II

BP 12.22: Poster

Dienstag, 2. April 2019, 14:00–16:00, Poster B2

Probing mitochondrial dynamics and heterogeneity during cell state switching using multiplexed, environment-sensitive fluorescent dyes — •Sufi O. Raja^{1, 2}, Gandhi Sivaraman¹, Christoph F. Schmidt², and Akash Gulyani¹ — ¹Technology for Advancement of Science, Institute for Stem Cell Biology & Regenerative Medicine, Bellary Road, Bangalore-560065, India — ²Drittes Physikalisches Institut - Biophysik, Fakultät für Physik, Georg-August-Universität Göttingen, 37077 Göttingen

Mitochondria are known as power house of the cell also play significant role in regulating cellular metabolism, calcium and ROS signaling as well as in programmed cell death. Despite decades on research, precise and real-time information on mitochondrial dynamics and functionality, is still limiting.To better visualize the functional dynamics of mitochondrion, we have developed red-emitting, multi-functional, novel mitochondrial probes that are sensitive to local environment, specifically parameters like micro-viscosity, pH, ROS, etc. The developed dyes have low toxicity and very high photo-stability, allowing their use in long term imaging. These dyes have yielded new insights into mitochondrial dynamics in embryonic stem cells as well as during onset of differentiation. In a different example, we have also used our new dyes to probe mitochondrial heterogeneity within primary *activated* cells. These results would be placed in the context of our larger efforts to build new ways of probing *cellular dynamics* with a focus on physico-chemical changes in the intra-cellular compartments.