Regensburg 2019 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 12: Poster II

BP 12.23: Poster

Dienstag, 2. April 2019, 14:00–16:00, Poster B2

In-line DNA optical mapping in nanochannels for biomedical applications — •Franziska Esmek — Center for Hybrid Nanostructures

On-chip DNA optical mapping allows studying intact individual molecules with higher throughput than conventional sequencing techniques. In optical mapping, typically a "fluorescent barcode-like" pattern is first created, then the DNA molecules are elongated and the fluorescent signal is read out to get information about the genomic structure for biomedical applications. We explore a technique to selectively label the DNA molecules with organic fluorophores as well as by competitive binding at specific locations of interest. The DNA molecules are then stretched in nanofluidic devices made by nanoimprint lithography in a two-minute process. The signal is read out in real time in a home made confocal set up. We perform in-line detection of DNA molecules as they pass through the nanochannels with a focused laser as point excitation and a photon counter, without using a camera. In this configuration, the molecules are detected as step-like peaks in time scans, allowing for real time read-out, with high throughput. Peak analysis (as intensity and duration) gives information about the molecule length, as well as its sequence-dependent barcode. Here there is no limitation with the length of the molecules, or the resolution due to the thermal drift and molecule movement.