Regensburg 2019 – scientific programme

Parts | Days | Selection | Search | Updates | Downloads | Help

BP: Fachverband Biologische Physik

BP 12: Poster II

BP 12.40: Poster

Tuesday, April 2, 2019, 14:00–16:00, Poster B2

3D-Printed Microfluidic Chip to Study Protein Organization in a Lipid Bilayer — •Sevde Puzda, Ralf Seemann, and Jean-Baptiste Fleury — Saarland University, Saarbruecken, Germany

We developed a layout for a 3D microfluidic chip whose master can be fabricated by state of the art 3D-Printing. The microfluidic chip allows to form, in a quasi-automatic manner, a free-standing lipid bilayer. The bilayer is produced by contacting two water droplets in an oil phase, where lipids molecules have been dispensed. Interestingly, this method does not require any microfluidic pumps (volume, or pressure). The bilayer formation is demonstrated by electrophysiological measurements and optical investigations with a normal view direction onto the bilayer. Proteins are reconstitute in this bilayer, and their dynamical self-organization properties are study in-situ.