Regensburg 2019 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 12: Poster II

BP 12.5: Poster

Dienstag, 2. April 2019, 14:00–16:00, Poster B2

Metal Induced Energy Transfer reveals nanostructure of an focal adhesion complex — •Fabian Port, Lydia Rebehn, and Kay-E. Gottschalk — Institute of Experimental Physics, Ulm University, Germany

Cell adhesion to the extracellular matrix does not only function as an anchor, it also enables cells to sense their environment [1]. The focal adhesion complex, which is responsible for these adhesions, is a complex structure consisting of a multitude of different proteins. Despite this important role its structure remains difficult to resolve [2]. Knowing the exact position of these proteins in the focal adhesion complex is necessary to understand the sensing mechanisms of the cell. For a detailed analysis of the focal adhesions, a method to measure small distances in cells is needed. A technique which meets this challenge is Metal Induced Energy Transfer (MIET) [3]. Here we show a first analysis of the distance between various focal adhesion proteins and the underlying surface in different cell lines and demonstrate the usefulness of MIET for analyzing molecular structures close to the basal membrane with nm accuracy in life cells.

[1] Geiger, B., Spatz, J. P., & Bershadsky, A. D., Nature Reviews. Molecular Cell Biology, 10(1), 21-33 (2009)

[2] Kanchanawong, P., Shtengel, G., Pasapera, A. M., Ramko, E. B., Davidson, M. W., Hess, H. F., & Waterman, C. M., Nature, 468(7323), 580-584 (2010)

[3] Chizhik, A. I., Rother, J., Gregor, I., Janshoff, A., & Enderlein, J., Nature Photonics, advance on(January), 1-8 (2014)