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BP: Fachverband Biologische Physik
BP 12: Poster II
BP 12.59: Poster
Tuesday, April 2, 2019, 14:00–16:00, Poster B2
Interaction of superparamagnetic iron oxide nanoparticles and transferrin — •Ulrike Martens1, Ali Abou-Hassan2, and Mihaela Delcea1 — 1Institute for Biochemistry/ZIK HIKE, Greifswald University, Germany — 2Laboratoire PHENIX, Sorbonne Université, Paris, France
Recent research demonstrated that nanoparticles (NPs) can enhance or suppress the immune response by binding to proteins of the blood stream. One important blood protein is transferrin which is composed of two distinct domains each containing an iron binding site. Its main role is to deliver iron to all biological tissues. By applying various biophysical techniques we investigated the interactions of superparamagnetic iron oxide nanoparticles (SPIONs) including maghemite γ-Fe2O3 and magnetite Fe3O4 with different coatings (e.g. citrate, chitosan) with transferrin. SPIONs present many advantages related to their magnetic properties, including magnetic manipulation and separation. The tools used allowed the characterization of the functionalized NP surface and the identification of structural changes of the proteins (e.g. circular dichroism spectroscopy) upon interaction with nanoparticles. In particular, dynamic light scattering measurements as well as SDS-PAGE revealed the corona formation of our model protein transferrin on the NPs, while transferrin also acted as a stabilizing agent of the colloidal suspension as verified by zeta potential measurements. In addition, the influence of the NPs on the iron binding site of transferrin in comparison to the binding to iron-free transferrin (apotransferrin) was studied via UV-Vis spectroscopy and urea PAGE.