Regensburg 2019 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 12: Poster II
BP 12.66: Poster
Dienstag, 2. April 2019, 14:00–16:00, Poster B2
Narrow escape: How long does it take for a camel to go through the eye of a needle? — •Elisabeth Meiser1, Reza Mohammadi2, Nicolas Vogel2, and Susanne Fenz1 — 1University of Würzburg, Biocenter: Cell and Developmental Biology, Würzburg, Germany — 2Friedrich-Alexander University Erlangen-Nürnberg, Institute of Particle Technology, Erlangen, Germany
The narrow escape problem (NEP) is a common problem in biology and biophysics. It deals with Brownian particles confined to a given domain with reflecting borders and only a small escape window. The mean first passage time of the particle can be calculated analytically for diffusion in two and three dimensions in several geometries. We aim to systematically test the solution of the NEP in two dimensions with micro-patterned planar model membranes. Micro-patterned membranes were produced by a lithography-based method to achieve patterned glass followed by vesicle fusion. Two lithography methods were tested: UV- and colloid-lithography. UV-lithography relies on a UV-cross-linkable resist to produce a pattern of hydrophilic and hydrophobic regions on the substrate by selective illumination. Colloid-lithography is alternative approach to prepare particular small membrane patches (d=1um). It exploits the shadowing effect of polymeric microspheres whilst coating the substrate with a thin layer of gold to achieve a structured gold film. After appropriate functionalization of the gold, membranes will only form on the bare glass. We will present our first results on membrane patterning and diffusion in solid supported lipid bilayers on the single-molecule level.