Regensburg 2019 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 14: Cell mechanics I

BP 14.11: Vortrag

Mittwoch, 3. April 2019, 12:30–12:45, H10

Stretching Adherent Cells with Light — •Tobias Neckernuss, Daniel Geiger, Jonas Pfeil, and Othmar Marti — Institute of Experimental Physics, Ulm University

In natural science and medicine mechanical properties of cells are important parameters. Over the years, countless techniques to assess all kinds of parameters have been developed for all kinds of cells. The most important ones are: stiffness, creep and relaxation constants. However, the investigation often relies on the interaction of a probe with the cells. Hence, the measured properties are always those of the joint system cell and probe. In 2001 Guck et al. demonstrated that it is possible to investigate the mechanical properties of suspended cells in a microfluidic channel with laser light. We present a setup to determine the mechanical properties of adherent cells in their physiological environment without having to alter their biochemistry or influence the measurement results by interaction of a probe with the cells. A setup for stretching and a method to detect the deformation of the cell have been developed together with the necessary data analysis algorithms. The deformation data are fitted to different kinds of viscoelastic models to describe the behavior of the cells with networks of springs and dashpods. The best models are selected by methods of information theory and the properties of 3T3 fibroblasts measured as cultured are compared to latrunculin treated ones.