Regensburg 2019 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 14: Cell mechanics I

BP 14.12: Vortrag

Mittwoch, 3. April 2019, 12:45–13:00, H10

A microfluidic method to sort capsules and cells according to their size and deformability — •Doriane Vesperini^{1, 2} and Anne Le Goff¹ — ¹Sorbonne universités, Université de technologie de Compiègne, CNRS, UMR 7 338 Biomécanique et Bioingénierie — ²INM, Leibniz Institute for New Materials, Cytoskeletal fibers, ( F. Lautenschläger Group)

Cell mechanical properties depend on their functions or pathologies such as cancer or infections. Sorting cells according to their stiffness is thus particularly interesting to help diagnosis. We propose a microfluidic device that consists of a half-cylinder obstacle located at the end of a rectangular straight channel. Upstream of the obstacle, a flow-focusing module centers cells on the obstacle. Downstream of the obstacle, a diffuser ends on 5 symmetrical outlets. Trajectories in the diffuser depend on several parameters, such as cell size, deformability and the capillary number Ca. The capillary number expresses the competition between elastic and viscous forces when the micro-object is squeezed onto the obstacle. Large and stiff micro-objects are more deflected than small and soft ones. We first demonstrate the efficiency of our device to sort micro-capsules according to their size at low flow strength and to their deformability at high flow strength. It is a passive, non-destructive, and sensitive system. We then downscale the microfluidic device in order to sort non-adherent cells.