Regensburg 2019 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 2: Membranes and vesicles I (joint session BP/CPP)

BP 2.6: Vortrag

Montag, 1. April 2019, 11:30–11:45, H10

The interaction of viral fusion peptides with model lipid membranes at high hydrostatic pressure — Göran Surmeier¹, Michael Paulus¹, Susanne Dogan¹, Yury Forov¹, Mirko Elbers¹, Simon Egger² und •Julia Nase¹ — ¹Fakultät Physik/DELTA, TU Dortmund, 44221 Dortmund — ²Physikalische Chemie, TU Dortmund, 44221 Dortmund

When a virus enters a host cell, the insertion of viral fusion peptides (FPs) into the target membrane catalyzes the membrane fusion reaction. We investigated the interaction of different FPs with model membranes in X-ray reflectivity measurements at the interface between monoolein/water mixtures and a silicon substrate. In addition, the bulk and interfacial structures were investigated with small angle X-ray scattering in transmission and in grazing incidence. Monoolein/water mixtures have a very rich pressure-dependent phase diagram. Notably, the inverse bicontinuous cubic phases exhibit structural analogies to the hemifusion intermediates. We found that pressurization triggers formation of ordered lamellar monoolein multilayers close to the interface even in a pressure range where the bulk material is in the cubic phase. Previous studies demonstrated the effect of FPs on the pressure-dependent phase boundaries [1]. We resolved the vertical membrane structure of some multilayers and monitored the penetration of FPs into the membrane. Experiments were performed in a custom-made high hydrostatic pressure cell [2] at beamlines ID31 of the ESRF and BL9 of DELTA. [1] A. Levin et al, J Phys Chem B 121 (2017) [2] F.J. Wirkert et al, J. Synchr. Radiat. 21 (2014)