Regensburg 2019 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 23: Biomaterials and biopolymers I (joint session BP/CPP)

BP 23.7: Vortrag

Donnerstag, 4. April 2019, 11:15–11:30, H10

Mechanical properties of UV-irradiated collagen fibrils studied with atomic force microscopy — •Marcus Schulze, Melanie Rogge, and Robert Stark — Physics of Surfaces, Materialwissenschaften, TU Darmstadt, Alarich-Weiss-Straße 16, 64287 Darmstadt

Collagen is a widely used component for the synthesis of substrates in the field of Tissue Engineering (TE). Cell adhesion and proliferation on theses substrates is strongly dependent on their mechanical properties which makes a controlled adjustment of these properties a key requirement for a more elaborated substrate design. Among several approaches, the irradiation of the collagen-based substrates with UV light proved itself a valuable technique to modify the mechanics without introducing cytotoxicity. For the evaluation of the influence of UV light on the mechanical properties of collagen fibrils in a liquid environment the atomic force microscope was used. Varying combinations of UV light sources (UV-A, UV-B, and UV-C) and fluids (deionized water and phosphate buffered saline (PBS)) were applied to two kinds of samples. The indentation modulus was measured on surface supported fibrils and a tensile modulus was derived from bending experiments on freely suspended collagen fibrils. Results suggested an increase in modulus within 30 minutes of treatment with UV-B or UV-C light in PBS.