Regensburg 2019 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 23: Biomaterials and biopolymers I (joint session BP/CPP)
BP 23.8: Vortrag
Donnerstag, 4. April 2019, 11:30–11:45, H10
The effect of surface functionalization and pH on protein-gold nanoparticle interactions — •Brahmaiah Meesaragandla1,2, Isabel Garcia3, Luis.M Liz-Marzán3,4, and Mihaela Delcea1,2 — 1Institute of Biochemistry, University of Greifswald, Greifswald, Germany — 2ZIK HIKE, University of Greifswald, Greifswald, Germany — 3CIC biomaGUNE and CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), San Sebastián, Spain — 4Ikerbasque, Basque Foundation for Science, Bilbao, Spain
In this work, we have investigated the interactions between differently functionalized gold nanoparticles (citrate, PEG-OMe, PEG-COOH, PEG-NH2, and glycan coated AuNPs) and human serum albumin (HSA) with pH using UV-Vis absorption, dynamic light scattering (DLS), and circular dichroism (CD) spectroscopy techniques. HSA exhibit different isomeric forms and undergoes conformational changes at different pH conditions (e.g. pH 3.8, 7.4, and 9.3). Both UV-Vis and DLS measurements have indicated the formation of protein corona. CD spectroscopy studies suggested that HSA conjugated to AuNPs undergoes a change in the secondary structure (decrease in alpha-helix) at various pH for all functionalized AuNPs. This change in protein secondary structure might be due to the type of dominant interaction between NPs and HSA (i.e electrostatic, hydrogen bonding). Our results indicated that both, surface charge and pH of the medium, influences the changes in HSA structure.