Regensburg 2019 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 23: Biomaterials and biopolymers I (joint session BP/CPP)
BP 23.9: Vortrag
Donnerstag, 4. April 2019, 11:45–12:00, H10
Temperature dependence of the elastic modulus of vapor deposited phospholipid bilayers on solid substrates — Maria J. Retamal1, •Rodrigo Catalan2, Marcelo Cisternas2, Nicolas Moraga2, Diego Diaz2, Tomas P. Corrales3, Mark Busch4, Patrick Huber4, Marco Soto-Arriaza1, and Ulrich G. Volkmann2 — 1Faculty of Chemistry and CIEN-UC, P. Univ. Catolica de Chile, Santiago, Chile — 2Institute of Physics and CIEN-UC, P. Univ. Catolica de Chile, Santiago, Chile — 3Department of Physics, UTFSM, Valparaiso, Chile — 4TUHH, Hamburg, Germany
Phospholipid membranes (PMs) play a key role in most physiological processes. Besides the function of membrane proteins, changes in the fluidity of the phospholipid membrane are crucial in the permeability of certain molecules, such as oxygen or glucose. We analyze with Atomic Force Microscopy (AFM) and Surface Force Spectroscopy (SFS) the temperature dependence of Young's modulus (YM) of non-functional PMs (DPPC, DMPC and DSPC). Phospholipids were vapor-deposited in high vacuum onto silicon substrates. AFM measurements in liquid confirm the self-assembly of the phospholipid bilayer and YM measurements with SFS indicate the main transitions of the phospholipid bilayers. We show that PMs made by PVD in high vacuum preserve their structure and mechanical properties after proper hydration. This study opens new pathways to assemble phospholipid mixtures by means of solvent-free membrane formation. Acknowledgements: FONDECYT Nos. 3160803 (MJR), 1180939 (UGV), 1171047 (MSA) and 11160664 (TPC), CONICYT Fellowship (MC) and CONICYT-PIA ACT 1409.