Regensburg 2019 – scientific programme
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BP: Fachverband Biologische Physik
BP 24: Cell adhesion and migration, multicellular systems I
BP 24.10: Talk
Thursday, April 4, 2019, 12:15–12:30, H11
Parallelized Manipulation of Single Cell Behaviour with Magnetic Nanoparticles and Micromagnetic Arrays — Koceila Aïzel1, •Cornelia Monzel1,2, Elie Balloul1, Chiara Vicario1, Loïc Toraille3, João Sampaio4, Stanislas Rohart4, Nicolas Vernier5, Mathieu Coppey1, Loïc Rondin3, Jean-François Roch3, and Maxime Dahan1 — 1Laboratoire Physico-Chimie, Institut Curie, Paris, France — 2Experimental Medical Physics, Heinrich-Heine Univ., Düsseldorf, Germany — 3Laboratoire Aimé Cotton, CNRS, Univ. Paris-Sud, ENS Cachan, Orsay, France — 4Laboratoire de Physique des Solides, CNRS, Univ. Paris-Sud, Univ. Paris-Saclay, Orsay, France — 5Centre de Nanosciences et de Nanotech., CNRS, Univ. Paris-Sud, Univ. Paris-Saclay, Orsay, France
The spatial manipulation of functionalized magnetic nanoparticles (MNPs) on subcellular scales is a powerful approach to probe and actuate biological processes in cells. In order to realize the manipulation of MNPs in a remote and well-defined manner, micromagnets are placed in the vicinity of the cell. The magnetic fields generated by these micromagnetic cuboids are quantified using optical magnetometry. Here, the spin properties of NV color centers in diamond enable determination of mT magnetic field distributions with micrometer sensitivity. We then arrange the micromagnets in arrays surrounded by cells, to realize a parallelized high-throughput manipulation. Using these arrays, we show that MNPs are efficiently redistributed in multiple cells and that functionalized MNPs can activate smallGTPases of cell signalling pathways.