Regensburg 2019 – wissenschaftliches Programm

Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe

BP: Fachverband Biologische Physik

BP 6: Poster I

BP 6.17: Poster

Montag, 1. April 2019, 17:30–19:30, Poster B2

Tailor-made single electrode device to study human stem cell derived neurons — •Jeremy Teuber — Center for Hybrid Nanostructures

Studying the electrophysiological properties of cells is a basic requirement for understanding the nature of electrical impulses between single neurons. We have designed and fabricated tailor-made single electrode devices on silicon wafers for electrophysiological measurements of individual cells. The bilayer electrode system on a silicon wafer was prepared by UV laser photolithography and chemical vapor deposition. Here, the electrode consists of a conducting gold layer where the electrical supply lines are covered with an insulating layer of silicon dioxide. Alignment and material deposition of the custom-made device were examined by light microscopy as well as scanning electron microscopy. Stable and robust bonding of the individual material layers ensures that the device endures a cleaning procedure with trypsin and EDTA to enable multiple usability of the device. A 3D-printed ring glued with PDMS to the wafer is used to form a defined volume, in which the neurons are confined during the cultivation forming neural networks on the electrode. In detail, human stem cells are stimulated by using glutamate or patch clamping and the generation of the action potential is measured by the substrate electrode. The devices are further optimized with respect to geometry and materials to obtain increased signal-to-noise ratios. This device offers the opportunity to gain deep understanding of the characteristics of stem cells derived neurons.