Regensburg 2019 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 6: Poster I

BP 6.23: Poster

Montag, 1. April 2019, 17:30–19:30, Poster B2

Functionalized lipid bilayers as a platform to study cell adhesion — •Anastasia Svetlova, Vanessa Maybeck, Jana Ellieroth, Frano Milos, and Andreas Offenhäusser — Institute of Bioelectronics (ICS-8), Forschungszentrum Juelich, Wilhelm-Johnen Straße, 52425 Juelich, Germany

Artificial lipid bilayer is the closest possible model for the cell membrane. Despite that, current methods of lipid bilayer assembly and functionalization do not provide a satisfactory mimic of cell-cell contact due to the inability to recreate an asymmetrical multicomponent system. In the current work, a method to produce an integrated solid-supported lipid bilayer combining natural extracts from cell membranes and artificially made lipid vesicles is proposed. This simple method allows delivery of transmembrane proteins and components of the extracellular matrix into the substrate. Physical properties such as lateral diffusion coefficient of lipids in the bilayer can be controlled by adjusting the ratio of components in the substrate. Biocompatibility of the composite natural/artificial lipid bilayers is evaluated by their interactions with the cardiomyocyte-like HL-1 cell line. Compared to fully artificial mixes, composite bilayers allow cells to adhere and develop a morphologically more normal cytoskeleton.