Regensburg 2019 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe
BP: Fachverband Biologische Physik
BP 6: Poster I
BP 6.31: Poster
Montag, 1. April 2019, 17:30–19:30, Poster B2
The nanomorphology of osteoblasts with regard to adhesion parameters investigated by Scanning Ion Conductance Microscopy — •Christian Völkner1, Regina Lange1, Martina Grüning2, Ingo Barke1, Barbara Nebe2, and Sylvia Speller1 — 1University of Rostock, Institute of Physics, 18051 Rostock — 2University Medical Center Rostock, Dept. of Cell Biology, 18057 Rostock
Our aim is to elucidate the behavior of osteoblasts (MG-63) and mechanisms of cell adhesion on material surfaces. This we address by investigating the nanomorphology of the cells on various substrate surfaces by means of Scanning Ion Conductance Microscopy (SICM). The cell membrane surfaces exhibit protrusions with leave-like shapes. We attribute these features to so called ruffles [1]. They were found to be very mobile on the cell surface, dominating the signal of membrane height fluctuations. Latter may be developed as a parameter to monitor cell activity. We also focus on the rim of the cells, which exhibits heights between 100 nm up to micrometers with respect to the underlying substrate surface with its own physico-chemical characteristic. This implies large gaps between the cell and the substrate in the periphery, indicating locally varying adhesion clefts.
[1] Chhabra et al., Nature Cell Biol. 9, 1110 (2007)