Regensburg 2019 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 6: Poster I

BP 6.32: Poster

Montag, 1. April 2019, 17:30–19:30, Poster B2

Influence of substrate elasticity and geometry on cell migration — •Stefanie Haberlandt and Florian Rehfeldt — Georg-August-University Goettingen, Third Institute of Physics - Biophysics, Friedrich-Hund-Platz 1, 37077 Goettingen, Germany

Cell migration plays an important role in many processes which are vital for the development and homeostasis of multi-cellular organisms. It is also a crucial aspect in the development of several diseases such as cancer. Since the various tissues in our body differ significantly with respect to mechanical properties, biochemical composition, and topography, a systematic investigation of how these micro-environments impact migration is essential. This will lead to a better understanding of e.g. invasive cancer cells during tumor metastasis. Here, we experimentally observe and analyze unconfined 2D migration of NIH-3T3 fibroblasts and hMSCs on collagen-I coated polyacrylamide (PA) gels of different Young's moduli E. Using a micro-patterning approach we also created distinctly confined areas on the elastic gels to analyze the impact of geometry on migration. Since patterning on soft gels is challenging, we compared different approaches and present data from migration analysis that depends on cell type, Young's modulus E, and geometry.