Regensburg 2019 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 6: Poster I

BP 6.34: Poster

Montag, 1. April 2019, 17:30–19:30, Poster B2

Comparison of mechanical properties of cells using different cantilevers as probe tips for atomic force microscopy — •Florian Frederick Stuckmann, Hsiao-Ching Tsai, and Mathias Getzlaff — Institut für Angewandte Physik, Heinrich-Heine-Universität, Düsseldorf, Deutschland

The elasticity is one of the most important characteristics of the cell. To determine concerning deviations caused by pathologies, atomic force microscopy with a precise cantilever represents a powerful tool.

This scientific work analyzed mechanical properties of fibroblasts. Using a spherical or a pyramidal cantilever the cell elasticity of the fibroblasts can be determined. After the application of the AFM-Mode 'Force-Mapping' and the following data process, values of the Young Modulus of the fibroblasts can be obtained, which are illustrated in a map. Hence, the elasticity of typical areas such as the cell perimeter or above the nucleus can be quantified.

As a consequence of the tip shape, performance differences between the two cantilever types can be observed when working with biological samples. Because elasticity values cannot be determined at every point in case of the pyramidal cantilever, the sharp tip seems to damage the cell membrane.