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BP: Fachverband Biologische Physik
BP 6: Poster I
BP 6.39: Poster
Monday, April 1, 2019, 17:30–19:30, Poster B2
Size-dependent forces during phagosomal transport — Simon Wieland1,2, •David Gitschier1,2, Magdalena Haaf1, Solange Hoffbauer1, and Holger Kress1 — 1Biological Physics Group, Department of Physics, University of Bayreuth, Germany — 2Joint first authors
The intracellular transport of organelles plays an important role for a large variety of cellular processes, such as exocytosis and endocytosis. It is well established that the transport of organelles is biochemically regulated. However recently, it was shown that also the size of the organelles has a strong influence on the transport. In macrophages it was found that large phagosomes are transported very persistently towards the nucleus whereas small phagosomes show a highly irregular Motion[1]. To unravel the molecular causes of this behavior, we investigated the intracellular transport forces of phagosomes as a function of their size by using magnetic tweezers. We found that transport forces increase monotonically for organelle sizes up to four micrometers. The scaling of the transport forces with the organelle sizes together with an identification of the types and numbers of involved motors can lead to a more fundamental understanding of intracellular transport and the cooperation of molecular motors.
[1] S. Keller, K. Berghoff, H. Kress, Phagosomal transport depends strongly on phagosome size. Scientific Reports, 7 (2017), 17068