Regensburg 2019 – wissenschaftliches Programm

Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe

BP: Fachverband Biologische Physik

BP 6: Poster I

BP 6.40: Poster

Montag, 1. April 2019, 17:30–19:30, Poster B2

CAOS - How to Stretch Adherent Cells — •Tobias Neckernuss, Daniel Geiger, Jonas Pfeil, and Othmar Marti — Institute of Experimental Physics, Ulm University

We demonstrate a new method to stretch adherent cells with light. This has the advantage that no interaction with a probe is necessary to determine the mechanical properties of cells. Additionally not only point like forces can be applied, but the cell is stretched over its whole membrane surface. We show measurements on 3T3 cells as cultured and compare them to measurements taken on 3T3 cells treated with latrunculin. The deformation data is fitted to viscoelastic models consisting of networks of springs and dashpods. With the Akaike singular information criterion the best models are selected. Results confirm that the new technique works well an delivers results in agreement with literature. Additionally, by investigation of the behavior of individual parameters conclusions about different parts of the cytoskeleton can be drawn. In addition, the new technique proved to be more sensitive and precise than the well established technique of passive microrheology.