Regensburg 2019 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 6: Poster I
BP 6.52: Poster
Montag, 1. April 2019, 17:30–19:30, Poster B2
Near Real Time Analysis of Stress Fiber Formation in Stem Cells — •Lara Hauke1, Carina Wollnik1, Benjamin Eltzner2, Stefan Huckemann2, and Florian Rehfeldt1 — 1Third Institute of Physics, Biophysics, Georg-August-University Göttingen — 2Institute of Mathematical Stochastic, Georg-August-University Göttingen
Human mesenchymal stem cells (hMSC) can be directed to differentiate into various lineages by different matrix elasticities. While changes in lineage specific protein expression occur over a period of days to weeks, significantly different structures of stress fibers are observable within the first 24 hours of plating [1] quantified by an order parameter S. With our massively parallel live-cell imaging set-up we record cells under physiological conditions (37 ∘C, 5 %CO2) over a period of 24-48 hours to obtain a statistically sufficiently large data set. We aim for a full representation of filament processes over time and space allowing for statistical analysis. This unbiased classification will be represented by persistence in space and time and potential cross-talk with other cytoskeletal components. For this we developed the FilamentSensor [2,3] a freely available tool for near real-time image analysis of stress fibers. We present experimental data where we can distinguish the development of hMSCs on 1 kPa, 10 kPa and 30 kPa elastic substrates with 99 % confidence and are working on single filament tracking and better analysis of orientation fields.
References: [1]A. Zemel, et al., Nat. Phys., 2010. [2]www.filament-sensor.de [3]B. Eltzner, et al., PLoS One, 2015.