Regensburg 2019 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe
BP: Fachverband Biologische Physik
BP 6: Poster I
BP 6.61: Poster
Montag, 1. April 2019, 17:30–19:30, Poster B2
Dynamic properties of actin cortex depend on the adhesion state of cells. — •Kevin Kaub1,2, Emmanuel Terriac1, and Franziska Lautenschläger1,2 — 1INM, Saarbrücken, Germany — 2Saarland University, Saarbrücken, Germany
The actin cortex plays an important role in mechanical stability and migration. In recent years new information on the structural and the dynamical properties of the actin cortex have been obtained but their interplay and their link to mechanical properties is still poorly understood. For adhered cells it has been shown that there are two essential processes that constitute the dynamics of the cortex. These processes are linked to the polymerisation mechanisms of actin filaments: one, fast, is induced by Arp2/3, while the other, slower, is induced by formins. Furthermore it has been established that these processes contribute to the overall dynamics in different proportions. Finally, the activity of the molecular motor myosin II is another factor to take into account in order to link the structure and the mechanical properties. In the case of suspended cells, there have been conflicting reports on the role of myosin activity in regards to the stiffness.
It is our goal to understand this contradiction. To that aim, we analyzed the dynamical properties of the actin cortex by the use of FRAP (Fluorescence recovery after photobleaching). We further assessed the influence of myosin, Arp2/3 and formin inhibition in different states of adhesion. Our results shows that the dynamical properties of the actin cortex are adhesion-state specific.