Regensburg 2019 – scientific programme
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BP: Fachverband Biologische Physik
BP 6: Poster I
BP 6.62: Poster
Monday, April 1, 2019, 17:30–19:30, Poster B2
Vimentin intermediate filaments rings deform nucleus during the first steps of adhesion — •Emmanuel Terriac1, Susanne Schütz2, and Franziska Lautenschläger1,2 — 1Leibniz Institute for New Materials, Saarbrücken, Germany — 2Faculty NT, Saarbrücken, Germany
The role of vimentin intermediate filament in different cellular processes, such as cell migration or cellular mechanics has been increasingly studied during the last decade.
Here, we show an unreported phenotype during the first hours of adhesion: filamentous vimentin is found in close vicinity of the cell nucleus and occasionaly, nuclei-deforming vimentin rings could be observed. We show that the accumulation of filamentous vimentin is decreasing over time as a function of the adhesion affinity of the cell for the substrate. Most structures are dissolved within the first 6 hours after depositing the cells over the surface which may be the reason of why these structures are not widely reported yet.
We propose that upon forced detachment of cells by conventional techniques such as trypsinization, cells are not able to control the depolymerization of vimentin while this step is controlled during mitosis. Due to the entanglement of the nucleus within the vimentin fibers the nucleus is deformed during spreading, potentially caused by the stretching of the entire vimentin network. This deformation is released once vimentin filaments get slowly dissolved and recycled during full adhesion. The implication of the strong applied forces on the nucleus via this mechanism remains to be investigated in future.