Regensburg 2019 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 6: Poster I
BP 6.79: Poster
Montag, 1. April 2019, 17:30–19:30, Poster B2
Quantifying non-equilibrium nuclear shape fluctuations — •Heidi Somsel1, Federico Gnesotto3, Chase P. Broedersz3, and Christoph F. Schmidt1,2 — 1Drittes Physikalisches Institut - Biophysik, Fakultät für Physik, Georg-August-Universität Göttingen, 37077 Göttingen — 2Department of Physics, Duke University, Durham, NC 27708, USA — 3rnold-Sommerfeld-Center for Theoretical Physics and Center for NanoScience, Ludwig-Maximilians-Universität, München, Germany
Cells actively generate forces that lead to rapid fluctuations, slow shape changes, locomotion, and more. The cell nucleus also shows shape changes and fluctuations. The main driver of active dynamics in cells is the cytoskeleton, via actin and myosin. We focus on the nuclear envelope to analyze nuclear non-equilibrium dynamics . We use fluorescent labeling of the nuclear lamina to visualize the fluctuations of this membrane. The fluctuations are analyzed using Broken Detailed Balance analysis (BDB), a technique to reveal and quantify non-equilibrium dynamics in steady-state systems. BDB is non-invasive, meaning that it does not require chemical or mechanical perturbations, which are difficult to perform in the cell nucleus.