Regensburg 2019 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 6: Poster I

BP 6.82: Poster

Montag, 1. April 2019, 17:30–19:30, Poster B2

Probing mesoscopic dynamics in the developing Drosophila embryo using fluorescent carbon nanotubes — •Constantin Kohl¹, Zhiyi Lv², Jörg Großhans², and Christoph F. Schmidt^{1, 3} — ¹Drittes Physikalisches Institut, Georg-August-Universität, 37077 Göttingen, Germany — ²Institut für Entwicklungsbiochemie, Universitätsmedizin Göttingen, 37077 Göttingen, Germany — ³Department of Physics, Duke University, Durham, NC 27708, USA

Semiconducting near-infrared (NIR) fluorescent carbon nanotubes (CNTs) are promising markers for in vivo studies. In this project, we use NIR fluorescent CNTs as markers for in vivo studies in Drosophila embryos. The photostable and intermittency-free NIR fluorescence of CNTs allows us to capture high frequency information of individual CNT trajectories in the whole developing organism over multiple cell cycles. We have built a setup, allowing for simultaneously imaging of CNT NIR fluorescence and nuclear His-GFP fluorescence. This enables us to capture intracellular dynamics on multiple time scales. We have combined the high frequency CNT NIR signals with the corresponding low frequency nuclear His-GFP signals. We have computed correlations of individual CNT trajectories in different fly types and in different cell cycle phases to characterize particular dynamics. Furthermore, we use NIR fluorescent CNTs in conjunction with particle image velocimetry (PIV) to capture the dynamics of the cytoplasmic flow in the developing Drosophila embryos.