Regensburg 2019 – scientific programme
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BP: Fachverband Biologische Physik
BP 7: Bioimaging and biospectroscopy II
BP 7.6: Talk
Tuesday, April 2, 2019, 10:45–11:00, H4
Single particle tracking in 2+1 Dimensions using interferometric scattering Microscopy — •Philipp Kellner1, Francesco Reina2, Christoffer Lagerholm2, and Christian Eggeling1,2 — 1Institute of Applied Optics and Biophysics, Philosophenweg 7, 07743 Jena — 2Weatherall Institute of Molecular Medicine University of Oxford, Headley Way, Oxford
Directly recording Tracks of moving particles is essential for various parts of physics. In modern biophysics measuring the diffusion of lipids and proteins on biological membrane model systems is an example. Those measurements reveal not only information about the moving particle itself but also about heterogenities in the surrounding structures. Such observations require high spatial and temporal resolution. Novel interferometric scattering Microscopy (iScat) provides localization precision in the nm-range and time-resolution down to several microseconds. This talk will present the basic principles of iScat-Microscopy and underline its usability for biological questions. Further, optical improvement of the setup and the possiblity of detection using small labels in biological model membrane systems will be highlighted.