Regensburg 2019 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 7: Bioimaging and biospectroscopy II
BP 7.9: Vortrag
Dienstag, 2. April 2019, 12:00–12:15, H4
Ratiometric fluorescence imaging and marker-free motion tracking of Langendorff-perfused beating rabbit hearts — •Vineesh Kappadan1,2, Johannes Schröder-Schetelig1,2, Ulrich Parlitz1,2,3, Stefan Luther1,2,3,4,5, and Jan Christoph1,3 — 1Max Planck Institute for Dynamics and Self-Organization, Göttingen, Germany — 2Institute for Nonlinear Dynamics, Georg-August-Universität, Göttingen, Germany — 3German Center for Cardiovascular Research (DZHK e.V.), partner site Göttingen, Germany — 4Institute of Pharmacology and Toxicology, University Medical Center Göttingen, Göttingen, Germany — 5Department of Physics and Department of Bioengineering, Northeastern University, Boston, USA
Optical mapping based on fluorescence imaging is used for visualizing membrane voltage and Ca2+ concentration of isolated, Langendorff-perfused intact hearts. Despite recent progress in imaging of isolated beating hearts, accurate measurements of quantities such as the duration of action potentials are still challenging due to residual motion artifacts present in the fluorescence signal. Motion artifacts can be significantly reduced by the electromechanical uncoupler Blebbistatin, which may, however, affect properties like the action potential duration (APD). We show that marker free motion tracking combined with ratiometric fluorescence imaging techniques can be used to more reliably measure the APD from Langendorff-perfused, beating rabbit hearts and we use this technique to investigate the effects of Blebbistatin on the APD and restitution properties of the heart.