Regensburg 2019 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 8: Cytoskeletal filaments
BP 8.2: Vortrag
Dienstag, 2. April 2019, 09:45–10:00, H10
Cytoskeletal organization within polar cells and interactions between different components — •Carsten Baltes1,2, Emmanuel Terriac1, and Franziska Lautenschläger1,2 — 1Leibniz-Institut für neue Materialen — 2Universität des Saarlandes
The cytoskeleton is composed of three main parts: actin filaments, microtubules and intermediate, like for in this study, vimentin. The organization of the cytoskeleton and shape of cells are linked. Here we investigate the effect of imposing shape on the cytoskeletal organization. We further disturb particular cytoskeletal parts in this setup to test their interactions between different filaments. To acquire those defined shapes we used micropatterns of fibronectin achieved with the Primo device (Alvéole Paris). We focused on two circular shapes, full circles as example for a symmetric shapes and 3/4 circle (which resemble the shape of PacMan) to enforce polarity to the cell. Using withaferin A, blebbistatin and rho-associated-protein kinase (ROCK) inhibitor Y27632, we selectively disturbed vimentin organization and myosin II activity. We observed effects on the distribution of the cytoskeletal elements. We further found different behavior between cells treated with blebbistatin and Y27632, leading to the hypothesis that ROCK may be essential in the structure and dynamic of the vimentin network. Our results help to understand the cytoskeletal architecture in cells and the link between the different networks. Our method might be useful for further investigations in the cytoskeletal interplay.