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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 17: Poster Session I

CPP 17.6: Poster

Montag, 1. April 2019, 17:30–19:30, Poster B1

Towards biomimetic carbon nanomembranes — •Raphael Dalpke¹, Anna Dreyer², Thorsten Seidel², André Beyer¹, Karl-Josef Dietz², and Armin Gölzhäuser¹ — ¹Physics of Supramolecular Systems and Surfaces, Faculty of Physics, Bielefeld University, Universitätsstraße 25, 33615 Bielefeld, Germany. — ²Biochemistry and Physiology of Plants, Faculty of Biology, Bielefeld University, Universitätsstraße 25, 33615 Bielefeld, Germany.

Cell membranes are a prerequisite for life. Incorporated membrane proteins facilitate the transfer of substances. To explore the mechanisms of vectorial transport across cell membranes, an ultrathin, biomimetic system is desirable which allows the controlled integration of proteins. Here, we present different strategies to immobilize proteins with high affinity on carbon nanomembranes (CNMs). Covalently bonded aromatic precursor molecules form self-assembled monolayers (SAMs) on metal surfaces. After the irradiation with low energy electrons (100 eV) these cross-link to mechanically stable, chemically inert, and permeable CNMs with a typical thickness of around 1 nm which can be transferred onto any arbitrary substrate for further usage [1]. In particular, we discuss functionalization strategies for reversible as well as for irreversible protein immobilization. The basis of the reversible type is the non-covalent interaction of streptavidin and biotin which can be released by d-desthiobiotin. The irreversible type is based on the maleimide coupling chemistry, where a stable thioether bond is formed.

[1] A. Turchanin and A. Gölzhäuser, Carbon Nanomembranes, Advanced Materials, 2016, 28 (29), pp. 6075-6103.