Regensburg 2019 – wissenschaftliches Programm
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CPP: Fachverband Chemische Physik und Polymerphysik
CPP 42: Plasmonics IV (joint session O/CPP)
CPP 42.9: Vortrag
Mittwoch, 3. April 2019, 17:00–17:15, H8
Surface-enhanced infrared absorption spectroscopy for in-vitro detection of the polypeptide monolayer conformation on a single gold nanoantenna — •Rostyslav Semenyshyn1,2, Florian Mörz1,2, Frank Neubrech1,3, and Harald Giessen1,2 — 14th Physics Institute and Research Center SCoPE, University of Stuttgart — 2Center for Integrated Quantum Science and Technology, IQST — 3Kirchhoff Institute for Physics, University of Heidelberg
In our present work, we demonstrate the capability of surface-enhanced infrared absorption spectroscopy for ultra-sensitive in-vitro detection of the secondary structure of polypeptides monolayer. We utilized a monolayer of thiols as a bonding spacer for deposition of poly-L-lysine (PLL) molecules onto the gold surface [1]. We tuned the length of nanoantennas to be resonant at the frequency of the amide-I band of PLL and performed SEIRA measurements in the D2O environment using a specially designed transmittance flowcell. To measure an optical response of a single nanostructure, we pushed our system towards the diffraction limit and therefore applied a light source with higher brilliance, namely specifically designed broadband tunable laser [2]. Then, we compare our results to a globar, as well as a synchrotron, which are well-known FTIR light sources. With further advances it might become possible to scale the process down to a few or even single proteins.
R. Semenyshyn, M. Hentschel, C. Stanglmair, T. Teutsch, C. Tarin, C. Pacholski, H. Giessen, and F. Neubrech, Nano Lett. (2018); doi: 10.1021/acs.nanolett.8b02372 [2] T. Steinle, F. Mörz, A. Steinmann, and H. Giessen, Opt. Lett. 41, 4863 (2016)