Regensburg 2019 – scientific programme
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CPP: Fachverband Chemische Physik und Polymerphysik
CPP 64: Active Matter III (joint session BP/CPP/DY)
CPP 64.8: Talk
Friday, April 5, 2019, 11:15–11:30, H11
Out-of-plane beating components of active axonemes isolated from Chlamydomonas reinhardtii — Azam Gholami1, •Soheil Mojiri2, Eberhard Bodenschatz1, and Jörg Enderlein2 — 1Max-Planck Instiute for Dynamics and Self-Organization, Göttingen, Germany — 2Georg-August Universität, Göttingen, Germany
Cilia and flagella are ubiquitous in the living world. They are essential for micro-scale driven transport of fluids or cells by cilia/flagellar beating. Their slender bodies are composed of a microtubule/molecular motor structure that when taken independently are called an axoneme. Axonemes move by bending waves that emerge from the interplay between internal stresses generated by dynein motor proteins . Here we use the novel multi-plane phase contrast imaging technique to record the three dimensional beating pattern of isolated axonemes from Chlamydomonas reinhardtii that beat in the vicinity of a substrate. We measure the torsion of the axoneme along the contour length with high spatiotemporal resolution. High precision information on out-of-plane beating component of axonemes allows us to check the validity of the resistive-force theory.