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DY: Fachverband Dynamik und Statistische Physik

DY 43: Anomalous diffusion / Brownian motion

DY 43.7: Vortrag

Donnerstag, 4. April 2019, 11:45–12:00, H3

Flat-to-curved transition during clathrin-mediated endocytosis — •Felix Frey¹, Delia Bucher², Kem A. Sochacki³, Susann Kummer², Jan-Philip Bergeest⁴, William J. Godinez⁴, Hans-Georg Kräusslich², Karl Rohr⁴, Justin W. Taraska³, Steeve Boulant², and Ulrich S. Schwarz¹ — ¹ITP, Heidelberg University — ²Department of Virology, University Hospital Heidelberg — ³NHLBI, NIH, Bethesda, U.S.A. — ⁴IPMB, Heidelberg University

Biological cells constantly transport material and information across their plasma membrane. The most important pathway for the uptake of nutrients and receptors is clathrin-mediated endocytosis (CME). Although CME has been studied for decades, the exact sequence of molecular and structural events remains elusive. Two basic models have been suggested for the way CME proceeds, namely the constant curvature and the constant area models [1]. Here, we combine correlative electron and light microscopy, super-resolution microscopy, single particle tracking and simple mathematical grow laws to estimate the temporal sequence of ultrastructural rearrangements of the clathrin coat. Our quantitative analysis shows that neither model is correct and that clathrin-coated structures initially grow flat but start to acquire curvature when on average 70% of the final clathrin content is reached [2]. [1] Haucke, Volker, and Michael M. Kozlov. "Membrane remodeling in clathrin-mediated endocytosis." J. Cell Sci. (2018), 131(17):jcs216812. [2] Bucher, Delia, et al. "Clathrin-adaptor ratio and membrane tension regulate the flat-to-curved transition of the clathrin coat during endocytosis." Nat. Commun. (2018), 9(1):1109.