Regensburg 2019 – wissenschaftliches Programm

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MM: Fachverband Metall- und Materialphysik

MM 15: Topical session (Symposium MM): Correlative and in-situ Microscopy in Materials Research

MM 15.1: Topical Talk

Dienstag, 2. April 2019, 10:15–10:45, H44

Correlative Microscopy of Biological Cells and Tissues by Scanning X-ray Diffraction, Holography, Tomography and Super-Resolution Optical Microscopy — Marten Bernhardt¹, Jan-David Nicolas¹, Andrew Wittmeier¹, Michael Sprung¹, Sarah Köster², and •Tim Salditt¹ — ¹Georg-August-Universität Göttingen — ²DESY, Hamburg

We present a correlative microscopy approach , which combines holographic x-ray imaging, x-ray scanning diffraction, and super-resolution optical fluorescence microscopy, in particular stimulated emission depletion (STED)-microscopy [1], implemented in the same synchrotron endstation. This allows us to image both labeled and unlabeled molecular components in biophysical and soft matter samples in a quasi-simultaneous scheme. We exploit the complementary contrast mechanisms of x-ray microscopy and optical fluorescence for studies of heart muscle cells: While scanning x-ray diffraction and x-ray holographic imaging provide structural information on the native electron-density distribution within the cell, fluorescent markers visualize specific molecular components.

[1] M. Bernhardt, J.-D. Nicolas, M. Osterhoff, H. Mittelstädt, M. Reuss, B. Harke, A. Wittmeier, M. Sprung, S. Köster, T. Salditt. Nature Communications 9: 3641 (2018)