Regensburg 2019 – scientific programme
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MM: Fachverband Metall- und Materialphysik
MM 19: Miscellaneous: Biomaterials, Magnetic Shape Memory Alloys, Sensors and Actuators (joint session MM/MA)
MM 19.5: Talk
Tuesday, April 2, 2019, 15:15–15:30, H46
Platform for quantitative analysis of biochemical processes in droplets using nano-sensors. — •Dmitry Belyaev1, Larysa Baraban1,2, and Gianaurelio Cuniberti1,2 — 1Institute for Materials Science TU Dresden, MBZ, Budapester Str. 27, 01069, Dresden, Germany — 2Technische Universität Dresden Center for Advancing Electronics Dresden, 01062, Dresden, Germany
Real time monitoring of bio-chemical reactions and processes, e.g. related to the cancer development is highly relevant. This, can be done by implementing miniaturized lab-on-a-chip detecting systems, incorporating microfluidics and Si nanowire field effect transistor (SiNW FET) chip allowing droplet analysis[1]. Here, the chemical reaction of β-galactosidase and ortho-nitrophenol-galactose (ONPG) was detected in a label free format, and the kinetics was analyzed using SiNW EFT. SiNW FET is ion sensitive device and it is able to detect presence of charged molecules or ions in the liquid environment. The reaction was analyzed in a numerous emulsion droplets generated in a microfluidic flow-cell, by means of honeycomb shaped NW FET chip. The flow cell was produced via combination of laser, UV and soft lithography techniques and consists of the droplets generation module and the channel structure with width of about 300μm, and is successfully integrated on silicon chip. We attribute the shift of the Isd current to the change on ionic composition of the media. Comparative data proved successful detection of the reaction.
1)J. Schütt et al., Nano Lett. 2016, 16, 8, 4991-5000