Regensburg 2019 – wissenschaftliches Programm

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O: Fachverband Oberflächenphysik

O 72: Poster Wednesday: Scanning Probe Techniques

O 72.1: Poster

Mittwoch, 3. April 2019, 17:45–20:00, Poster B2

The Use of Spherical Particles for Cantilevers in an Atomic Force Microscope — •Katja Stommel, Hsiao-Ching Tsai, and Mathias Getzlaff — Institut für Angewandte Physik, Heinrich-Heine Universität Düsseldorf, Germany

The elasticity of cells is an important property and mainly influenced by the cell's cytoskeleton. With an AFM it is possible to measure the elasticity of soft materials. It is necessary to use a probe tip with a known, well-defined shape to determine the Young Modulus of, e.g., cells using the Hertz model. Hence, the goal of this project is to test and document a reproducible method of attaching a spherical particle to a cantilever.

A combined system including an AFM and a transmitted light-microscope is used for the process, during which a spherical particle of silica is glued at a tipless cantilever. To control the quality of the modified cantilever, another transmitted light-microscope is used. Images showing the side and the top view are acquired and used to determine the size and shape of the attached particles. The quality check shows that the method to modify cantilevers cannot be reproduced easily. Because the imaging is not precise enough, the conditions of the Hertz model are not necessarily fulfilled.