Regensburg 2019 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe
ST: Fachverband Strahlen- und Medizinphysik
ST 1: X-ray Imaging
ST 1.8: Vortrag
Montag, 1. April 2019, 17:00–17:15, H48
Hematein-based X-ray suitable Stain enables 3D Visualization of the Cell Nuclei — •Katharina Scheidt1, Mark Müller1, Melanie A. Kimm2, Simone Ferstl1, Sebastian Allner1, Klaus Achterhold1, Julia Herzen1, Franz Pfeiffer1,2, and Madleen Busse1 — 1Department of Physics and Munich School of Bioengineering, TU Munich, Germany — 2Department of Diagnostic and Interventional Radiology, Klinikum rechts der Isar, TU Munich, Germany
The current gold standard for the investigations of anatomical structures on a cellular level is histology which is limited to two-dimensional imaging and often shows disruption of the cell architecture due to the sectioning of the tissue. In contrast to that, non-destructive 3D imaging methodologies such as X-ray microscopic and nanoscopic CT struggle with the weak attenuation of soft tissue hampering their application in virtual 3D histology. The presented nucleus-specific X-ray stain provides a visualization of anatomical structures in three dimensions whereby it addresses specifically the cell nuclei. With this technique, the real 3D morphology and the spatial distribution of cell nuclei is maintained. Together with a newly developed laboratory nanoscopic CT system, 3D visualization in nanometer range is possible. Furthermore, the staining technique is compatible with conventional 2D histology as microscopic slides can be derived from the very same stained soft-tissue sample and further counter staining is possible.[1]
[1] M.Müller, A.M.Kimm et al., Non-destructive high-resolution 3D virtual histology enabled through a cell nucleus-specific stain for X-ray computes tomography. Sci. Rep., 2018, manuscript accepted.