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MS: Fachverband Massenspektrometrie

MS 6: Clusters and Molecules

MS 6.2: Hauptvortrag

Mittwoch, 13. März 2019, 14:30–15:00, U A-Esch 2

Hitting proteins with a sledgehammer – mass spectrometry meets X-rays — •Charlotte Uetrecht — Heinrich Pette Institute, Hamburg, Germany — European XFEL, Schenfeld, Germany

Native ion mobility mass spectrometry (MS) is a perfect tool to study protein complexes in a mass and conformation specific manner, albeit with low structural resolution. On the other hand, it allows monitoring structural transitions, which cannot be purified and are inaccessible for crystallography. The European XFEL, the world’s most intense hard X-ray free-electron laser (XFEL), has just become operational and offers an opportunity to obtain high resolution structures of single particles. The benefits of native MS for single particle imaging of transient intermediates at European XFEL and initial feasibility studies will be presented. Furthermore, we employed soft X-rays from synchrotrons and FELs for fragmentation in native MS. Our recent results show great potential for using soft X-rays in native top-down MS. The available intensity could alleviate proposed upper size limits for UV photo dissociation of protein complexes.